Human Pyruvate Dehydrogenase Alpha (PDHa) ELISA Kit

Human Pyruvate Dehydrogenase Alpha (PDHa) ELISA Kit

Short Description:

  • FOB Price: Inquiry
  • Min.Order Quantity: 48T
  • Supply Ability: 500 kits/Month
  • Port: Shanghai
  • Payment Terms: T/T in advance
  • Product Detail

    Product Tags

    Product name: Human Pyruvate Dehydrogenase Alpha (PDHa) ELISA Kit
    Method: sandwich

    PDHCE1A; PHE1A; PHE1-A; PDHA1; PDH-A; Pyruvate Dehydrogenase(lipoamide)Alpha 1; Pyruvate Dehydrogenase E1 Component Subunit Alpha,Aomatic Form,Mitochondrial

    Catalog number: DL-PDHa-Hu
    Detection range: 0.156-10ng/mL
    Size: 96T
    Assay length 1-4.5Hours
    Price: inquiry
    Quality guarantee period: for 12 months

    Human Pyruvate Dehydrogenase Alpha (PDHa) ELISA Kitelisa kit elisa kits

    Item Standard Test

    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PDHa in human serum, plasma, tissue homogenates and other biological fluids.

    Identification Colorimetric Positive
    Composition Pre-coated, ready to use 96-well strip plate 1 Conform
    Standard (freeze dried) 2
    Standard Diluent 1 × 20ml
    Detection Reagent A 1× 120μl
    Detection Reagent B 1× 120μl
    Assay Diluent A 1 × 12ml
    Assay Diluent B 1 × 12ml
    TMB Substhumane 1 × 9ml
    Stop Solution 1 ×6ml
    Wash Buffer(30 x concenthumane) 1 ×20ml
    Plate sealer for 96 wells 2
    Instruction manual 1
    Test principle

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.


    Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.

    Matrix Recovery range (%) Average(%)
    serum(n=5) 81-93 86
    EDTA plasma(n=5) 80-97 88
    heparin plasma(n=5) 90-101 95

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    Sample 1:2 1:4 1:8 1:16
    serum(n=5) 82-96% 83-98% 81-99% 93-101%
    EDTA plasma(n=5) 88-101% 86-95% 90-102% 80-93%
    heparin plasma(n=5) 80-91% 82-90% 95-104% 79-95%

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%


    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
    To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

    Assay procedure summary

    1. Prepare all reagents, samples and standards;
    2. Add 100μL standard or sample to each well. Incubate 2 hours at 37℃;
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37℃;
    4. Aspirate and wash 3 times;
    5. Add 100μL prepared Detection Reagent B. Incubate 1 hour at 37℃;
    6. Aspirate and wash 5 times;
    7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37℃;
    8. Add 50μL Stop Solution. Read at 450nm immediately.

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