elisa kit elisa kits logo
search elisa kits
elisa kit contact phone ico

Tel:+86-510-82732223
Fax:+86-510-82715101-8014
Email: info@dldevelop.com.cn

Home > Polyclonal & monoclonal

Rabbit anti-ABCB10 Antibody

0
 code image
Product name: Rabbit anti-ABCB10 Antibody
Catalog:

DL91576A

Synonyms : ATP-binding cassette sub-family B member 10, mitochondrial; ATP-binding cassette transporter 10; ABC transporter 10 protein; Mitochondrial ATP-binding cassette 2; M-ABC2
Reactivity: H, M, R
Applications: WB, IHC, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ABCB10. The exact sequence is proprietary.
WB description: Western blot analysis of ABCB10 expression in HeLa (A), mouse kidney (B), rat kidney (C) whole cell lysates.
IHC description: Immunohistochemical analysis of ABCB10 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:

Immunofluorescent analysis of ABCB10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene ID(Human): 23456
Gene ID (Mouse)): 56199
SwissProt (Human): Q9NRK6
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

Order or get a Quote

We will reply you within 24 hours!

Copyright @ Wuxi Donglin Sci & Tech Development Co.,Ltd. All Rights Reserved Elisa Kit|Elisa Kits Language:Chinese

苏ICP备06050612号-1 苏公网安备 32020302000048号