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Home > Polyclonal & monoclonal

Rabbit anti-SUMO1 Antibody

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Product name: Rabbit anti-SUMO1 Antibody
Catalog:

DL91206A

Synonyms : SMT3C; SMT3H3; UBL1; Small ubiquitin-related modifier 1; SUMO-1; GAP-modifying protein 1; GMP1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Smt3C; Ubiquitin-like protein UBL1
Reactivity: H, M, R, B, D, Mk, P
Applications: WB, IHC, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SUMO1. The exact sequence is proprietary.
WB description: Western blot analysis of SUMO1 expression in MCF7 (A), A431 (B), HeLa (C), mouse kidney (D), rat kidney (E) whole cell lysates.
IHC description: Immunohistochemical analysis of SUMO1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:

Immunofluorescent analysis of SUMO1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene ID(Human): 7341
Gene ID (Mouse)): 22218
Gene ID(Rat): 301442
SwissProt (Human): P63165
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

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