Rabbit anti-TNF alpha Antibody

Product name:	Rabbit anti-TNF alpha Antibody
Catalog:	DL91174A
Synonyms :	TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Reactivity:	H, M, R, Mk, P
Applications:	WB, IHC
Size:	50uL/100uL
Host:	Rabbit
Clonality:	Polyclonal
Concentration:	1mg/mL
Immunogen:	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNF alpha. The exact sequence is proprietary.
WB description:	Western blot analysis of TNF alpha expression in HeLa (A), NIH3T3 (B), mouse heart (C), rat brain (D) whole cell lysates.
IHC description:	Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Purification_Method:	The antibody was purified by immunogen affinity chromatography.
Buffer:	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:	WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene ID(Human):	7124
Gene ID (Mouse)):	21926
Gene ID(Rat):	24835
SwissProt (Human):	P01375
Storage:	Store at -20℃. Avoid repeated freeze / thaw cycles.

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DL91174A