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Home > Polyclonal & monoclonal

Rabbit anti-S6K1 Antibody

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Product name: Rabbit anti-S6K1 Antibody
Catalog:

DL91034A

Synonyms : STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
Reactivity: H, M, R, B, C, D, Mk, P, Rb
Applications: WB, IHC, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human S6K1. The exact sequence is proprietary.
WB description: Western blot analysis of S6K1 expression in MCF7 (A), HeLa (B), HEK293 (C), mouse brain (D) whole cell lysates.
IHC description: Immunohistochemical analysis of S6K1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:

Immunofluorescent analysis of S6K1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene ID(Human): 6198
Gene ID (Mouse)): 72508
Gene ID(Rat): 83840
SwissProt (Human): P23443
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

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