Rabbit anti-c-RAF Antibody

Product name:	Rabbit anti-c-RAF Antibody
Catalog:	DL90985A
Synonyms :	RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Reactivity:	H, M, R, B, C, P
Applications:	WB, IF/IC
Size:	50uL/100uL
Host:	Rabbit
Clonality:	Polyclonal
Concentration:	1mg/mL
Immunogen:	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-RAF. The exact sequence is proprietary.
WB description:	Western blot analysis of c-RAF expression in A431 (A), HeLa (B), RAW264.7 (C), C6 (D) whole cell lysates.
IF/ICC description:	Immunofluorescent analysis of c-RAF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Purification_Method:	The antibody was purified by immunogen affinity chromatography.
Buffer:	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:	WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Gene ID(Human):	5894
Gene ID (Mouse)):	110157
Gene ID(Rat):	24703
SwissProt (Human):	P04049
Storage:	Store at -20℃. Avoid repeated freeze / thaw cycles.

We will reply you within 24 hours!

DL90985A