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FAQ
Rabbit anti-eNOS (pS1177) Antibody
| Product name: | Rabbit anti-eNOS (pS1177) Antibody | |
| Catalog: | DL90818A |
|
| Synonyms : | Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII | |
| Reactivity: | H, M, R, B, D, P, Rb, S | |
| Applications: | WB, IF/IC, IP | |
| Size: | 50uL/100uL | |
| Host: | Rabbit | |
| Clonality: | Polyclonal | |
| Concentration: | 1mg/mL | |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human eNOS. The exact sequence is proprietary. | |
| WB description: | Western blot analysis of eNOS (pS1177) expression in A549 (A), HuvEc (B), rat brain (C) whole cell lysates. | |
| IF/ICC description: | Immunofluorescent analysis of eNOS (pS1177) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
|
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. | |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
| Dilution: | WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) | |
| Gene ID(Human): | 4846 | |
| Gene ID (Mouse)): | 18127 | |
| Gene ID(Rat): | 24600 | |
| SwissProt (Human): | P29474 | |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. | |
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