Rabbit anti-HSC70 Antibody

Product name:	Rabbit anti-HSC70 Antibody
Catalog:	DL90554A
Synonyms :	HSC70; HSP73; HSPA10; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; Lipopolysaccharide-associated protein 1; LAP-1; LPS-associated protein 1
Reactivity:	H, M, R, B, C, Mk, P
Applications:	WB, IF/IC, IP
Size:	50uL/100uL
Host:	Rabbit
Clonality:	Polyclonal
Concentration:	1mg/mL
Immunogen:	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.
WB description:	Western blot analysis of HSC70 expression in HeLa (A), NIH3T3 (B) whole cell lysates.
IF/ICC description:	Immunofluorescent analysis of HSC70 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Purification_Method:	The antibody was purified by immunogen affinity chromatography.
Buffer:	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:	WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Gene ID(Human):	3312
Gene ID (Mouse)):	15481
Gene ID(Rat):	24468
SwissProt (Human):	P11142
Storage:	Store at -20℃. Avoid repeated freeze / thaw cycles.

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DL90554A