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Home > Polyclonal & monoclonal

Rabbit anti-hnRNP K (pS284) Antibody

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Product name: Rabbit anti-hnRNP K (pS284) Antibody
Catalog:

DL90538A

Synonyms : HNRPK; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP
Reactivity: H, M, R, B, C, Rb, Z
Applications: WB, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human hnRNP K. The exact sequence is proprietary.
WB description: Western blot analysis of hnRNP K (pS284) expression in MCF7 EGF-treated (A), Raw264.7 EGF-treated (B), H9C2 EGF-treated (C) whole cell lysates.
IF/ICC description:

Immunofluorescent analysis of hnRNP K (pS284) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Gene ID(Human): 3190
Gene ID (Mouse)): 15387
Gene ID(Rat): 117282
SwissProt (Human): P61978
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

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