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Home > Polyclonal & monoclonal

Rabbit anti-DAXX Antibody

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Product name: Rabbit anti-DAXX Antibody
Catalog:

DL90332A

Synonyms : BING2; DAP6; Death domain-associated protein 6; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein
Reactivity: H, Mk
Applications: WB, IHC, IF/IC, IP
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DAXX. The exact sequence is proprietary.
WB description: Western blot analysis of DAXX expression in HeLa (A), Molt4 (B), Ramos (C), K562 (D), HEK293T (E) whole cell lysates.
IHC description: Immunohistochemical analysis of DAXX staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:

Immunofluorescent analysis of DAXX staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Gene ID(Human): 1616
SwissProt (Human): Q9UER7
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

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