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FAQ
Rabbit anti-AKT (pT308) Antibody
| Product name: | Rabbit anti-AKT (pT308) Antibody | |
| Catalog: | DL90032A |
|
| Synonyms : | AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT3; PKBG; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2 | |
| Reactivity: | H, M, R, B, C, Z | |
| Applications: | WB, IF/IC, IP | |
| Size: | 50uL/100uL | |
| Host: | Rabbit | |
| Clonality: | Polyclonal | |
| Concentration: | 1mg/mL | |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKT. The exact sequence is proprietary. | |
| WB description: | Western blot analysis of AKT (pT308) expression in HeLa colchicine-treated (A), HL60 (B), NIH3T3 (C), SP2/0 colchicine-treated (D), PC12 colchicine-treated (E) whole cell lysates. | |
| IF/ICC description: | Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
|
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. | |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
| Dilution: | WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) | |
| Gene ID(Human): | 207; 208; 10000 | |
| Gene ID (Mouse)): | 11651; 11652; 23797 | |
| Gene ID(Rat): | 24185; 25233; 29414 | |
| SwissProt (Human): | P31749; P31751; Q9Y243 | |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. | |
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