Acetylcholinesterase (AchE)
Acetylcholinesterase (AchE), also called true cholinesterase (AchE) : high activity, selective hydrolysis of Ach essential enzyme, can make Ach hydrolyzed into choline and acetic acid.The true choli-neesterase substrates in cholinesterase are highly specific because they only break down the narrow range of substrates centered on acetylcholine.Acetylcholinesterase is a key enzyme in biological nerve conduction. In the synapse of cholinergic synapse, this enzyme can degrade acetylcholine, terminate the excitatory effect of neurotransmitters on the postsynaptic membrane, and ensure the normal transmission of nerve signals in the organism.
Studies have shown that extracellular AchE can change the electrical activity of the substantia nigra dopamine neurons and the motor behavior of animals.In large doses, AchE inhibits the spontaneous discharge of the substantia nigra dopaminergic cells, but in physiological doses it increases the discharge frequency of the substantia nigra cells.Creenfield found by in vitro culture cell recording add AchE can cause the substantia nigra in perfusion cell membrane hyperpolarization significantly, accompanied by membrane input resistance is reduced, with the irreversibility of cholinesterase inhibitors Mr (soman) processing AchE in advance, and then add it to the brain slice medium, and can't change the AchE the electrical properties of the membrane.The above experiments show that AchE has an electrical effect on the dopaminergic neuron membrane of the substantia nigra, which is independent of AchE hydrolysis and has high structural specificity.
Microelectrode was used to inject AchE directly into the rat's substantia nigra, and the result was that the movement behavior of the rat was changed, which was slow and stiff.The behavior changes of the rats after an AchE injection lasted for a long time, and the mechanism is not clear.It is speculated that when AchE is released from the dendrites of the substantia nigra dopaminergic cell in the physiological state, it can exert certain influence on the physiological activities of the neuron itself and its neighboring cells, which may be a new way of local connection between neurons or self-feedback regulation.
https://dldevelop.com/Research-reagent/dl-ache-hu.html
https://dldevelop.com/Research-reagent/dl-ache-mu.html
https://dldevelop.com/Research-reagent/dl-ache-ra.html
Studies have shown that extracellular AchE can change the electrical activity of the substantia nigra dopamine neurons and the motor behavior of animals.In large doses, AchE inhibits the spontaneous discharge of the substantia nigra dopaminergic cells, but in physiological doses it increases the discharge frequency of the substantia nigra cells.Creenfield found by in vitro culture cell recording add AchE can cause the substantia nigra in perfusion cell membrane hyperpolarization significantly, accompanied by membrane input resistance is reduced, with the irreversibility of cholinesterase inhibitors Mr (soman) processing AchE in advance, and then add it to the brain slice medium, and can't change the AchE the electrical properties of the membrane.The above experiments show that AchE has an electrical effect on the dopaminergic neuron membrane of the substantia nigra, which is independent of AchE hydrolysis and has high structural specificity.
Microelectrode was used to inject AchE directly into the rat's substantia nigra, and the result was that the movement behavior of the rat was changed, which was slow and stiff.The behavior changes of the rats after an AchE injection lasted for a long time, and the mechanism is not clear.It is speculated that when AchE is released from the dendrites of the substantia nigra dopaminergic cell in the physiological state, it can exert certain influence on the physiological activities of the neuron itself and its neighboring cells, which may be a new way of local connection between neurons or self-feedback regulation.
https://dldevelop.com/Research-reagent/dl-ache-hu.html
https://dldevelop.com/Research-reagent/dl-ache-mu.html
https://dldevelop.com/Research-reagent/dl-ache-ra.html
