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Human Baculoviral IAP Repeat Containing Protein 6 (BIRC6) ELISA Kit
two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.
| Traditional ELISA Kit | Ready-to-Use ELISA KIT | |
| Product name: | Human Baculoviral IAP Repeat Containing Protein 6 (BIRC6) ELISA Kit | |
| Method: | Sandwich | |
| Synonyms: | BRUCE; Apollon; BIR repeat-containing ubiquitin-conjugating enzyme; Ubiquitin-conjugating BIR domain enzyme apollon |
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| Detection range: | 0.312-20ng/mL | |
| Target Protein: | BIRC6 | |
| Size: | 96T/48T | |
| Quality guarantee period: | for 12 months, 16 months | |
| Catalog number: | DL-BIRC6-Hu (traditional) | (ready-to-use) |
| Assay length | 1-4.5Hours | 1-3.5Hours |
| Advantages: |
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| Instruction Manual |
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Other names：BRUCE; Apollon; BIR repeat-containing ubiquitin-conjugating enzyme; Ubiquitin-conjugating BIR domain enzyme apollon
Function: Protects cells from undergoing apoptosis.
Sequence：
10 20 30 40 50 MVTGGGAAPP GTVTEPLPSV IVLSAGRKMA AAAAAASGPG CSSAAGAGAA 60 70 80 90 100 GVSEWLVLRD GCMHCDADGL HSLSYHPALN AILAVTSRGT IKVIDGTSGA 110 120 130 140 150 TLQASALSAK PGGQVKCQYI SAVDKVIFVD DYAVGCRKDL NGILLLDTAL 160 170 180 190 200 QTPVSKQDDV VQLELPVTEA QQLLSACLEK VDISSTEGYD LFITQLKDGL 210 220 230 240 250 KNTSHETAAN HKVAKWATVT FHLPHHVLKS IASAIVNELK KINQNVAALP 260 270 280 290 300 VASSVMDRLS YLLPSARPEL GVGPGRSVDR SLMYSEANRR ETFTSWPHVG 310 320 330 340 350 YRWAQPDPMA QAGFYHQPAS SGDDRAMCFT CSVCLVCWEP TDEPWSEHER 360 370 380 390 400 HSPNCPFVKG EHTQNVPLSV TLATSPAQFP CTDGTDRISC FGSGSCPHFL 410 420 430 440 450 AAATKRGKIC IWDVSKLMKV HLKFEINAYD PAIVQQLILS GDPSSGVDSR 460 470 480 490 500 RPTLAWLEDS SSCSDIPKLE GDSDDLLEDS DSEEHSRSDS VTGHTSQKEA 510 520 530 540 550 MEVSLDITAL SILQQPEKLQ WEIVANVLED TVKDLEELGA NPCLTNSKSE 560 570 580 590 600 KTKEKHQEQH NIPFPCLLAG GLLTYKSPAT SPISSNSHRS LDGLSRTQGE 610 620 630 640 650 SISEQGSTDN ESCTNSELNS PLVRRTLPVL LLYSIKESDE KAGKIFSQMN 660 670 680 690 700 NIMSKSLHDD GFTVPQIIEM ELDSQEQLLL QDPPVTYIQQ FADAAANLTS 710 720 730 740 750 PDSEKWNSVF PKPGTLVQCL RLPKFAEEEN LCIDSITPCA DGIHLLVGLR 760 770 780 790 800 TCPVESLSAI NQVEALNNLN KLNSALCNRR KGELESNLAV VNGANISVIQ 810 820 830 840 850 HESPADVQTP LIIQPEQRNV SGGYLVLYKM NYATRIVTLE EEPIKIQHIK 860 870 880 890 900 DPQDTITSLI LLPPDILDNR EDDCEEPIED MQLTSKNGFE REKTSDISTL 910 920 930 940 950 GHLVITTQGG YVKILDLSNF EILAKVEPPK KEGTEEQDTF VSVIYCSGTD 960 970 980 990 1000 RLCACTKGGE LHFLQIGGTC DDIDEADILV DGSLSKGIEP SSEGSKPLSN 1010 1020 1030 1040 1050 PSSPGISGVD LLVDQPFTLE ILTSLVELTR FETLTPRFSA TVPPCWVEVQ 1060 1070 1080 1090 1100 QEQQQRRHPQ HLHQQHHGDA AQHTRTWKLQ TDSNSWDEHV FELVLPKACM 1110 1120 1130 1140 1150 VGHVDFKFVL NSNITNIPQI QVTLLKNKAP GLGKVNALNI EVEQNGKPSL 1160 1170 1180 1190 1200 VDLNEEMQHM DVEESQCLRL CPFLEDHKED ILCGPVWLAS GLDLSGHAGM 1210 1220 1230 1240 1250 LTLTSPKLVK GMAGGKYRSF LIHVKAVNER GTEEICNGGM RPVVRLPSLK 1260 1270 1280 1290 1300 HQSNKGYSLA SLLAKVAAGK EKSSNVKNEN TSGTRKSENL RGCDLLQEVS 1310 1320 1330 1340 1350 VTIRRFKKTS ISKERVQRCA MLQFSEFHEK LVNTLCRKTD DGQITEHAQS 1360 1370 1380 1390 1400 LVLDTLCWLA GVHSNGPGSS KEGNENLLSK TRKFLSDIVR VCFFEAGRSI 1410 1420 1430 1440 1450 AHKCARFLAL CISNGKCDPC QPAFGPVLLK ALLDNMSFLP AATTGGSVYW 1460 1470 1480 1490 1500 YFVLLNYVKD EDLAGCSTAC ASLLTAVSRQ LQDRLTPMEA LLQTRYGLYS 1510 1520 1530 1540 1550 SPFDPVLFDL EMSGSSCKNV YNSSIGVQSD EIDLSDVLSG NGKVSSCTAA 1560 1570 1580 1590 1600 EGSFTSLTGL LEVEPLHFTC VSTSDGTRIE RDDAMSSFGV TPAVGGLSSG 1610 1620 1630 1640 1650 TVGEASTALS SAAQVALQSL SHAMASAEQQ LQVLQEKQQQ LLKLQQQKAK 1660 1670 1680 1690 1700 LEAKLHQTTA AAAAAASAVG PVHNSVPSNP VAAPGFFIHP SDVIPPTPKT 1710 1720 1730 1740 1750 TPLFMTPPLT PPNEAVSVVI NAELAQLFPG SVIDPPAVNL AAHNKNSNKS 1760 1770 1780 1790 1800 RMNPLGSGLA LAISHASHFL QPPPHQSIII ERMHSGARRF VTLDFGRPIL 1810 1820 1830 1840 1850 LTDVLIPTCG DLASLSIDIW TLGEEVDGRR LVVATDISTH SLILHDLIPP 1860 1870 1880 1890 1900 PVCRFMKITV IGRYGSTNAR AKIPLGFYYG HTYILPWESE LKLMHDPLKG 1910 1920 1930 1940 1950 EGESANQPEI DQHLAMMVAL QEDIQCRYNL ACHRLETLLQ SIDLPPLNSA 1960 1970 1980 1990 2000 NNAQYFLRKP DKAVEEDSRV FSAYQDCIQL QLQLNLAHNA VQRLKVALGA 2010 2020 2030 2040 2050 SRKMLSETSN PEDLIQTSST EQLRTIIRYL LDTLLSLLHA SNGHSVPAVL 2060 2070 2080 2090 2100 QSTFHAQACE ELFKHLCISG TPKIRLHTGL LLVQLCGGER WWGQFLSNVL 2110 2120 2130 2140 2150 QELYNSEQLL IFPQDRVFML LSCIGQRSLS NSGVLESLLN LLDNLLSPLQ 2160 2170 2180 2190 2200 PQLPMHRRTE GVLDIPMISW VVMLVSRLLD YVATVEDEAA AAKKPLNGNQ 2210 2220 2230 2240 2250 WSFINNNLHT QSLNRSSKGS SSLDRLYSRK IRKQLVHHKQ QLNLLKAKQK 2260 2270 2280 2290 2300 ALVEQMEKEK IQSNKGSSYK LLVEQAKLKQ ATSKHFKDLI RLRRTAEWSR 2310 2320 2330 2340 2350 SNLDTEVTTA KESPEIEPLP FTLAHERCIS VVQKLVLFLL SMDFTCHADL 2360 2370 2380 2390 2400 LLFVCKVLAR IANATRPTIH LCEIVNEPQL ERLLLLLVGT DFNRGDISWG 2410 2420 2430 2440 2450 GAWAQYSLTC MLQDILAGEL LAPVAAEAME EGTVGDDVGA TAGDSDDSLQ 2460 2470 2480 2490 2500 QSSVQLLETI DEPLTHDITG APPLSSLEKD KEIDLELLQD LMEVDIDPLD 2510 2520 2530 2540 2550 IDLEKDPLAA KVFKPISSTW YDYWGADYGT YNYNPYIGGL GIPVAKPPAN 2560 2570 2580 2590 2600 TEKNGSQTVS VSVSQALDAR LEVGLEQQAE LMLKMMSTLE ADSILQALTN 2610 2620 2630 2640 2650 TSPTLSQSPT GTDDSLLGGL QAANQTSQLI IQLSSVPMLN VCFNKLFSML 2660 2670 2680 2690 2700 QVHHVQLESL LQLWLTLSLN SSSTGNKENG ADIFLYNANR IPVISLNQAS 2710 2720 2730 2740 2750 ITSFLTVLAW YPNTLLRTWC LVLHSLTLMT NMQLNSGSSS AIGTQESTAH 2760 2770 2780 2790 2800 LLVSDPNLIH VLVKFLSGTS PHGTNQHSPQ VGPTATQAMQ EFLTRLQVHL 2810 2820 2830 2840 2850 SSTCPQIFSE FLLKLIHILS TERGAFQTGQ GPLDAQVKLL EFTLEQNFEV 2860 2870 2880 2890 2900 VSVSTISAVI ESVTFLVHHY ITCSDKVMSR SGSDSSVGAR ACFGGLFANL 2910 2920 2930 2940 2950 IRPGDAKAVC GEMTRDQLMF DLLKLVNILV QLPLSGNREY SARVSVTTNT 2960 2970 2980 2990 3000 TDSVSDEEKV SGGKDGNGSS TSVQGSPAYV ADLVLANQQI MSQILSALGL 3010 3020 3030 3040 3050 CNSSAMAMII GASGLHLTKH ENFHGGLDAI SVGDGLFTIL TTLSKKASTV 3060 3070 3080 3090 3100 HMMLQPILTY MACGYMGRQG SLATCQLSEP LLWFILRVLD TSDALKAFHD 3110 3120 3130 3140 3150 MGGVQLICNN MVTSTRAIVN TARSMVSTIM KFLDSGPNKA VDSTLKTRIL 3160 3170 3180 3190 3200 ASEPDNAEGI HNFAPLGTIT SSSPTAQPAE VLLQATPPHR RARSAAWSYI 3210 3220 3230 3240 3250 FLPEEAWCDL TIHLPAAVLL KEIHIQPHLA SLATCPSSVS VEVSADGVNM 3260 3270 3280 3290 3300 LPLSTPVVTS GLTYIKIQLV KAEVASAVCL RLHRPRDAST LGLSQIKLLG 3310 3320 3330 3340 3350 LTAFGTTSSA TVNNPFLPSE DQVSKTSIGW LRLLHHCLTH ISDLEGMMAS 3360 3370 3380 3390 3400 AAAPTANLLQ TCAALLMSPY CGMHSPNIEV VLVKIGLQST RIGLKLIDIL 3410 3420 3430 3440 3450 LRNCAASGSD PTDLNSPLLF GRLNGLSSDS TIDILYQLGT TQDPGTKDRI 3460 3470 3480 3490 3500 QALLKWVSDS ARVAAMKRSG RMNYMCPNSS TVEYGLLMPS PSHLHCVAAI 3510 3520 3530 3540 3550 LWHSYELLVE YDLPALLDQE LFELLFNWSM SLPCNMVLKK AVDSLLCSMC 3560 3570 3580 3590 3600 HVHPNYFSLL MGWMGITPPP VQCHHRLSMT DDSKKQDLSS SLTDDSKNAQ 3610 3620 3630 3640 3650 APLALTESHL ATLASSSQSP EAIKQLLDSG LPSLLVRSLA SFCFSHISSS 3660 3670 3680 3690 3700 ESIAQSIDIS QDKLRRHHVP QQCNKMPITA DLVAPILRFL TEVGNSHIMK 3710 3720 3730 3740 3750 DWLGGSEVNP LWTALLFLLC HSGSTSGSHN LGAQQTSARS ASLSSAATTG 3760 3770 3780 3790 3800 LTTQQRTAIE NATVAFFLQC ISCHPNNQKL MAQVLCELFQ TSPQRGNLPT 3810 3820 3830 3840 3850 SGNISGFIRR LFLQLMLEDE KVTMFLQSPC PLYKGRINAT SHVIQHPMYG 3860 3870 3880 3890 3900 AGHKFRTLHL PVSTTLSDVL DRVSDTPSIT AKLISEQKDD KEKKNHEEKE 3910 3920 3930 3940 3950 KVKAENGFQD NYSVVVASGL KSQSKRAVSA TPPRPPSRRG RTIPDKIGST 3960 3970 3980 3990 4000 SGAEAANKII TVPVFHLFHK LLAGQPLPAE MTLAQLLTLL YDRKLPQGYR 4010 4020 4030 4040 4050 SIDLTVKLGS RVITDPSLSK TDSYKRLHPE KDHGDLLASC PEDEALTPGD 4060 4070 4080 4090 4100 ECMDGILDES LLETCPIQSP LQVFAGMGGL ALIAERLPML YPEVIQQVSA 4110 4120 4130 4140 4150 PVVTSTTQEK PKDSDQFEWV TIEQSGELVY EAPETVAAEP PPIKSAVQTM 4160 4170 4180 4190 4200 SPIPAHSLAA FGLFLRLPGY AEVLLKERKH AQCLLRLVLG VTDDGEGSHI 4210 4220 4230 4240 4250 LQSPSANVLP TLPFHVLRSL FSTTPLTTDD GVLLRRMALE IGALHLILVC 4260 4270 4280 4290 4300 LSALSHHSPR VPNSSVNQTE PQVSSSHNPT STEEQQLYWA KGTGFGTGST 4310 4320 4330 4340 4350 ASGWDVEQAL TKQRLEEEHV TCLLQVLASY INPVSSAVNG EAQSSHETRG 4360 4370 4380 4390 4400 QNSNALPSVL LELLSQSCLI PAMSSYLRND SVLDMARHVP LYRALLELLR 4410 4420 4430 4440 4450 AIASCAAMVP LLLPLSTENG EEEEEQSECQ TSVGTLLAKM KTCVDTYTNR 4460 4470 4480 4490 4500 LRSKRENVKT GVKPDASDQE PEGLTLLVPD IQKTAEIVYA ATTSLRQANQ 4510 4520 4530 4540 4550 EKKLGEYSKK AAMKPKPLSV LKSLEEKYVA VMKKLQFDTF EMVSEDEDGK 4560 4570 4580 4590 4600 LGFKVNYHYM SQVKNANDAN SAARARRLAQ EAVTLSTSLP LSSSSSVFVR 4610 4620 4630 4640 4650 CDEERLDIMK VLITGPADTP YANGCFEFDV YFPQDYPSSP PLVNLETTGG 4660 4670 4680 4690 4700 HSVRFNPNLY NDGKVCLSIL NTWHGRPEEK WNPQTSSFLQ VLVSVQSLIL 4710 4720 4730 4740 4750 VAEPYFNEPG YERSRGTPSG TQSSREYDGN IRQATVKWAM LEQIRNPSPC 4760 4770 4780 4790 4800 FKEVIHKHFY LKRVEIMAQC EEWIADIQQY SSDKRVGRTM SHHAAALKRH 4810 4820 4830 4840 4850 TAQLREELLK LPCPEGLDPD TDDAPEVCRA TTGAEETLMH DQVKPSSSKE LPSDFQL
INTENDED USE
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of BIRC6 in human tissue homogenates, cell lysates or other biological fluids.
DETECTION RANGE
0.312-20ng/mL. The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL.
SENSITIVITY
The minimum detectable dose of BIRC6 is typically less than 0.107ng/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of BIRC6.
No significant cross-reactivity or interference between BIRC6 and analogues was observed.
You can reference link of the kit as following
https://dldevelop.com/Research-reagent/dl-birc6-hu.html
https://www.dldevelop.com/uploadfile/data/DL-BIRC6-Hu.pdf
Introduction
| Item | Standard | Test | |
| Description |
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of BIRC6 in human tissue homogenates, cell lysates or other biological fluids. |
Conform | |
| Identification | Colorimetric | Positive | |
| Composition | Traditional ELISA Kit | Ready-to-Use ELISA KIT | Conform |
| Pre-coated, ready to use 96-well strip plate 1 | Pre-coated, ready to use 96-well strip plate 1 | ||
| Plate sealer for 96 wells 2 | Plate sealer for 96 wells 2 | ||
| Standard 2 | Standard 2 | ||
| Diluents buffer 1×45mL | Standard Diluent 1×20mL | ||
| Detection Reagent A 1×120μL | Detection Solution A 1×12mL | ||
| Detection Reagent B 1×120μL | Detection Solution B 1×12mL | ||
| TMB Substrate 1×9mL | TMB Substrate 1×9mL | ||
| Stop Solution 1×6mL | Stop Solution 1×6mL | ||
| Wash Buffer (30 × concentrate) 1×20mL | Wash Buffer (30 × concentrate) 1×20mL | ||
| Instruction manual 1 | Instruction manual 1 | ||
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Recovery
Matrices listed below were spiked with certain level of recombinant BIRC6 and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-93 | 86 |
| EDTA plasma(n=5) | 80-97 | 88 |
| heparin plasma(n=5) | 90-101 | 95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1：2 | 1：4 | 1：8 | 1：16 |
| serum(n=5) | 82-96% | 83-98% | 81-99% | 93-101% |
| EDTA plasma(n=5) | 88-101% | 86-95% | 90-102% | 80-93% |
| heparin plasma(n=5) | 80-91% | 82-90% | 95-104% | 79-95% |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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